Recombinant IL-8 antibody

Catalog No. ABIN7543350

The Rabbit Chimeric anti-IL-8 antibody has been validated for IHC, ELISA and Neut. It is suitable to detect IL-8 in samples from Human.

Quick Overview for Recombinant IL-8 antibody (ABIN7543350)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This IL-8 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Neutralization (Neut)

Clone: WS-4

Product Details anti-IL-8 Antibody

Purpose: Anti-IL-8 [WS-4], Rabbit IgG, kappa

Specificity: This antibody binds human IL-8, which is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. This antibody cross reacts with rabbit IL-8.

Cross-Reactivity: Rabbit

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with human IL-8. This reshaped WS-4 antibody was generated by grafting CDRs of mouse monoclonal antibody onto human framework regions.

Isotype: IgG kappa

Application Details

Application Notes: This antibody was used in the generation of a very sensitive ELISA for the detection of human IL-8 in various body fluids. The original mouse version of this antibody has a detection limit of 16 pg/mL in an ELISA. This antibody can bind both the 72 and 77 amino acid forms of IL-8 produced by cultured human cells and cell lines stimulated with IL-1 (PMID: 1593135). The neutralization activity of this antibody towards IL-8 was studied in a rabbit model of severe acute pancreatitis. It was reported that this antibody can reduce the acute lung injury, presumably through inhibition of circulating IL-8 and TNF-alpha, and CD11b/CD18 in lung tissue (PMID: 10189850). This antibody can neutralize rabbit IL-8 in vivo (PMID: 8347559). The effect of neutralizing antibody on IL-8 enhanced infectious cytomegalovirus (CMV) production was examined and it was reported that this antibody reduced virus production nearly to unstimulated levels at 1,000 ng/mL (PMID: 7933146). This antibody was used in the immunohistochemical staining and immuno-localization of IL-8 in tissue sections taken from intestinal loops of patients infected with either M90T or BS176 strains of Shigella flexneri (PMID: 10024597).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for IL-8

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: IL-8

Background: IL8, Interleukin-8, CXCL8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, GCP-1, Monocyte-derived neutrophil chemotactic factor, MDNCF, Monocyte-derived neutrophil-activating peptide, MONAP, Neutrophil-activating protein 1, NAP-1, Protein 3-10C, T-cell chemotactic factor

UniProt: P10145

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy