Recombinant FAP antibody

Catalog No. ABIN7543357

The Mouse Monoclonal anti-FAP antibody (Clone 73-3) (ABIN7543357) specifically detects FAP in WB, IP, FACS, in vitro and in vivo. The antibody is reactive with Mouse samples.

Quick Overview for Recombinant FAP antibody (ABIN7543357)

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAP antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), In vitro Assay (in vitro), In vivo Studies (in vivo)

Clone: 73-3

Product Details anti-FAP Antibody

Purpose: Anti-FAP [73.3], Mouse IgG1, kappa

Specificity: The antibody is specific for fibroblast activation protein (FAP). FAP is a membrane protease that is highly expressed by cancer-associated fibroblasts (CAFs).

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing FAP-null mice with FAP-expressing 3T3 cells.

Isotype: IgG1 kappa

Application Details

Application Notes: A second-generation retroviral CAR, consisting of the scFv fragment of the antibody, with CD8 stalk, human CD3ζ and 4-1BB domains, targeting mouse FAP was developed. The Fap-CAR-T cells killed FAP-expressing 3T3 target cells specifically. The FAP-CAR-T cells were tested in vivo in three different established models, showing that the growth of tumors was significantly reduced by 35-50 % following treatment. Further, the anti-FAP-CAR T cells demonstrated enhanced antitumor response when combined with a tumor vaccine. No significant toxicity was observed in the treated mice. (Wang et al., 2014, PMID: 24778279) FAP+ cells were identified using flow cytometry of lung tissue using the antibody (Kimura et al., 2019, PMID: 31188013). This antibody was used for detection of murine FAP expression on HT1080 cells by flow cytometry (de Sostoa et al. 2019, PMID: 30683154). Immunoprecipitation of FAP was performed on lung humogenate using this antibody. The antibody detected FAP by western blot analysis (Fan et al., 2016, PMID: 26663085).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for FAP

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Alternative Name: FAP

Background: Fibroblast activation protein, Fibroblast activation protein-α, Prolyl endopeptidase FAP, Dipeptidyl peptidase FAP, Fibroblast activation protein alpha, FAPalpha1, Gelatine degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme,Serine integral membrane protease, SIMP, Surface-expressed protease, Seprase

UniProt: P97321

Pathways: Tube Formation