Recombinant CTLA4 antibody

Catalog No. ABIN7543362

The Rabbit Chimeric anti-CTLA4 antibody has been validated for ELISA and in vivo. It is suitable to detect CTLA4 in samples from Human.

Quick Overview for Recombinant CTLA4 antibody (ABIN7543362)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CTLA4 antibody is un-conjugated

Application: ELISA, In vivo Studies (in vivo)

Clone: 5E3

Product Details anti-CTLA4 Antibody

Purpose: Anti-CTLA-4 [5E3], Rabbit IgG, kappa

Specificity: The antibody is specific for CTLA-4. CTLA-4 is a protein receptor that functions as an immune checkpoint and downregulates immune responses.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with CTLA-4 protein.

Isotype: IgG kappa

Application Details

Application Notes: The specificity of the antibody was confirmed by ELISA analysis. The kinetic parameters of human CTLA-4 protein binding to the antibody were measured and they resulted to be at the sub-nm level. The antitumor activity of the humanized antibody was demonstrated in vivo in animal models. It was proved that the antibody inhibited the tumor in a dose-dependent manner. Mouse PD-1 antibody was combined with the antibody and used to evaluate the toxicity to mice, these experiments compared with the use of Ipilimumab in combination with PD-1 antibody. Results showed that after 5E3 antibody was used in combination with PD-1 antibody, the immunity to the body and the toxicity of heart, liver and lung are significantly lower than the toxicity of Ipilimumab in combination with PD-1 antibody (CN110343180).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CTLA-4

Background: Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CD152

UniProt: P16410

Pathways: Cancer Immune Checkpoints