Recombinant Dystroglycan antibody

Catalog No. ABIN7543384

This anti-Dystroglycan antibody is a Rabbit Chimeric antibody detecting Dystroglycan in ELISA and FACS. Suitable for Human.

Quick Overview for Recombinant Dystroglycan antibody (ABIN7543384)

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This Dystroglycan antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Clone: P38G1

Product Details anti-Dystroglycan Antibody

Purpose: Anti-Beta Dystroglycan [P38G1], Rabbit IgG, kappa

Specificity: This antibody recognizes human and mouse beta dystroglycan (β-DAG). Beta-dystroglycan is a transmembrane protein that plays an important role in connecting the extracellular matrix to the cytoskeleton. It plays an important role in linking dystrophin to the laminin-binding alpha-dystroglycan. It acts as a cell adhesion receptor in both muscle and non-muscle tissues. It also functions in cell adhesion-mediated signaling and implicated in cell polarity.

Cross-Reactivity: Mouse

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with a mixture of transfected HEK 293 cells expressing human and mouse beta-DAG.

Isotype: IgG kappa

Application Details

Application Notes: The binding specificity of this antibody to human and mouse beta dystroglycan expressed on the surface of CHO cells was confirmed using ELISA and flow cytometry. The binding affinity of the original IgG antibody to human beta dystroglycan was determined to be 941 pM. This antibody was used in combination with anti-Laminin antibody PL60B3 for the generation of a bispecific antibody (CN113651891).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Dystroglycan

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Alternative Name: Beta Dystroglycan

Background: Dystroglycan 1, Dag1, Beta-DG, β-DAG, BETA DAG, Dystroglycan, Dystrophin-associated glycoprotein 1, B-Dystroglycan, B Dystroglycan, Beta-dystroglycan, β dystroglycan, β-dystroglycan

UniProt: Q14118, Q62165

Pathways: Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus