Recombinant GFAP antibody

Catalog No. ABIN7543392

The Rabbit Chimeric anti-GFAP antibody has been validated for WB, IHC and ELISA. It is suitable to detect GFAP in samples from Human.

Quick Overview for Recombinant GFAP antibody (ABIN7543392)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 10G1G11H7

Product Details anti-GFAP Antibody

Purpose: Anti-GFAP [10G1G11H7], Rabbit IgG, kappa

Specificity: The original antibody recognizes the recombinant protein GFAP. GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with purified recombinant GFAP protein.

Isotype: IgG kappa

Application Details

Application Notes: The antibody specifically detected GFAP protein by Western blot analysis. This antibody and the commercial antibody 6F2 were used for immunohistochemistry on astrocytoma, glioma, pituitary and brain tissue and the results were compared. The antibody 10G1G11H7 had accurate staining location on the cell membrane, the staining was clear without non-specific staining, and the background was clean. Further, the results were consistent with the ones of the commercially available antibody GFAP (6F2), indicating that the specificity of the antibodies is equivalent (CN110903389A).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background: Glial fibrillary acidic protein, CGMCCNO.17407, 6F2

UniProt: P14136