Recombinant OCT4 antibody

Catalog No. ABIN7543401

The Rabbit Chimeric anti-OCT4 antibody has been validated for WB and IHC. It is suitable to detect OCT4 in samples from Human.

Quick Overview for Recombinant OCT4 antibody (ABIN7543401)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 41B1

Product Details anti-OCT4 Antibody

Purpose: Anti-Oct3/4 [41B1], Rabbit IgG, kappa

Specificity: The original antibody is specific for OCT3/4. OCT3/4 is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). It forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. It is critical for early embryogenesis and for embryonic stem cell pluripotency.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with recombinant protein OCT3/4.

Isotype: IgG kappa

Application Details

Application Notes: The antibody 41B1 and the commercial antibody C-10 were used to stain collagen tumor, dysgerminoma and testicular tissue in immunohistochemistry. Results showed the antibody specificity is comparable to the commercial antibody. Further, the staining intensity of the antibody 41B1 was higher than the commercial antibody (CN113072642A).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: Oct3/4

Background: C-10, KS20.8, POU5F1, Oct4, POU domain, class 5, transcription factor 1, POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, CGMCC NO 20779

UniProt: Q01860

Pathways: Stem Cell Maintenance