Recombinant TdT antibody

Catalog No. ABIN7543412

This Rabbit Chimeric antibody specifically detects TdT in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant TdT antibody (ABIN7543412)

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This TdT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 32D5

Product Details anti-TdT Antibody

Purpose: Anti-Terminal Deoxynucleotidyl Transferase [32D5], Rabbit IgG, kappa

Specificity: The antibody is specific for TdT. TdT catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with the recombinant TdT protein.

Isotype: IgG kappa

Application Details

Application Notes: The antibody specifically detected TDT protein in Western blot analysis. The antibody 32D5 and the commercial antibody SEN28 were used to stain lymphoma and thymus. Results showed the antibody specificity is comparable to the commercial antibody. Further, the sensitivity of the antibody 32D5 was higher than the commercial antibody. The antibody and the commercially available antibody TA347 were simultaneously detected on the normal tissue chip, indicating that the specificity of the antibody in normal tissue was equivalent to that of the commercially available antibody (CN113265003A).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for TdT

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Alternative Name: Terminal Deoxynucleotidyl Transferase

Background: TdT, DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal transferase, CGMCC NO 20777

UniProt: P04053

Pathways: DNA Damage Repair