Recombinant PD-1 antibody

Catalog No. ABIN7543420

This anti-PD-1 antibody is a Rabbit Chimeric antibody detecting PD-1 in FACS and ELISA. Suitable for Human.

Quick Overview for Recombinant PD-1 antibody (ABIN7543420)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This PD-1 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA

Clone: 1G4

Product Details anti-PD-1 Antibody

Purpose: Anti-PD-1 [1G4], Rabbit IgG, kappa

Specificity: This antibody binds human PD-1 protein. PD-1 acts as an inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. It delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2.

Cross-Reactivity: Rhesus Monkey

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG4

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing 6-8 weeks old female BALB/c mice with his-tagged human PD-1 protein.

Isotype: IgG kappa

Application Details

Application Notes: The initial binding characterization of the antibody was done using ELISA. The binding reactivity of this antibody to human, mouse and rhesus monkey PD-1 expressed on CHO cells was determined using flow cytometry. This antibody was shown to cross react with human and rhesus monkey PD-1 and not mouse PD-1. The binding affinity of the original mouse IgG4 antibody for human PD-1 was determined using surface plasmon resonance and was found to be Kd= 1.07 nM. The anti-tumor effect of this antibody was tested in vivo in a mouse colon cancer model. Murine MC-38 cells (colon adenocarcinoma cells) were injected subcutaneously in B-hPD-1 mice and treated with this antibody. It was reported that this antibody showed good tumor inhibitory effect (CN111763257).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG4 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: PD-1

Background: CD279, PD1, PD 1, hPD-1, PDCD1, Programmed cell death protein 1, Programmed cell death 1, Protein PD-1, 41-1G4, 1G4-mHvKv

UniProt: Q15116

Pathways: Cancer Immune Checkpoints