Recombinant HLA-DR10 antibody

Catalog No. ABIN7543426

The Rabbit Chimeric anti-HLA-DR10 antibody (Clone Lym-1) (ABIN7543426) specifically detects HLA-DR10 in IHC, WB, FACS, IF, IP, RIA and StM. The antibody is reactive with Human samples.

Quick Overview for Recombinant HLA-DR10 antibody (ABIN7543426)

Target: HLA-DR10

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Radioimmunoassay (RIA), Staining Methods (StM)

Clone: Lym-1

Product Details

Purpose: Anti-HLA-DR10 [Lym-1], Rabbit IgG, kappa

Specificity: This antibody has high affinity for a discontinuous epitope on the β subunit of the human HLA-DR10 surface membrane antigen of malignant B cells, which is not expressed on normal lymphocytes. HLA-DR10 (DR10) is a HLA-DR serotype that recognizes the DRB1*1001 gene product.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with tumor cell nuclei preparations as immunogens.

Isotype: IgG kappa

Application Details

Application Notes: This antibody could recognize cell lines of B-cell lineage but not cells of T-cell, myeloid, or erythroid derivation in a live cell radioimmunoassay. Flow cytometric analysis of 22 lymphoma biopsies showed that the majority of B-cell tumors were LYM-1 positive. Approximately 40 % of B-cell chronic lymphocytic leukemias were found to be positive with Lym-1. The binding of this antibody to peripheral blot lymphocytes was assessed using indirect immunofluorescence staining. Immunoperoxidase staining of nonlymphoid human organs and tissues revealed weak reactivity of Lym-1. This antibody was also capable of immunoprecipitating HLA-DR10 antigen from Raji cells and ARH-77 myeloma cells. (PMID: 3542194). This antibody also was used for immunohistochemical staining of advanced B-cell NHL tissues from patients (PMID: 10809213). This antibody can be used to identify the HLA-DR variant HLA-DR10 in a western blot (PMID: 8917632). LYM-1 chimeric antigen receptor T cells were demonstrated to exhibit potent anti-tumor effects against B-cell lymphoma (PMID: 29261129).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: HLA-DR10

Background: CD74, Beta chain HLA-DR, HLA-DR β-chain, β-chain, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii, p33

UniProt: P04233