Recombinant Apo-B100 antibody

Catalog No. ABIN7543445

This Mouse Monoclonal antibody specifically detects Apo-B100 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Recombinant Apo-B100 antibody (ABIN7543445)

Target: Apo-B100 (Apoprotein B100 (Apo-B100))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Apo-B100 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: MabB9

Product Details anti-Apo-B100 Antibody

Purpose: Anti-Apolipoprotein B-100 [MabB9], Mouse IgG2b, kappa

Specificity: This antibody binds human apolipoprotein B (APOB). Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. It is involved in the metabolism of lipids and is the main protein constituent of lipoproteins such as very low-density lipoprotein (VLDL) and low-density lipoprotein (LDL, the ""bad cholesterol"").

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing female BALB/c mice intraperitoneally with 20ug of human apo B-100 and dead Salmonella microbes.

Isotype: IgG2b kappa

Application Details

Application Notes: The binding characterization of this antibody towards human APO-B was done using ELISA and immunoblotting. This antibody was also used in a serum assay to determine the quantity of apo-B in a sample using competitive ELISA. This antibody was shown to react with isolated human Apo-B as well as low density lipids but not with high density lipids and lipoprotein-deficient serum (Yoon et al., 1993).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Apo-B100

Target: Apo-B100 (Apoprotein B100 (Apo-B100))

Alternative Name: Apolipoprotein B-100

Background: APOB, Apolipoprotein B-100, Apo B-100

UniProt: P04114