AIFM1 antibody (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1) (AA 130-180)

Details for Product anti-AIFM1 Antibody No. ABIN724010
Target
Binding Specificity
AA 130-180
19
14
11
9
5
5
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse, Pig, Rat
123
71
70
23
14
13
9
9
8
7
5
4
4
2
1
1
Host
Rabbit
111
12
2
2
Clonality
Polyclonal
Conjugate
This AIFM1 antibody is un-conjugated
7
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
99
48
41
29
29
19
12
11
10
8
5
2
2
1
1
Options
Immunogen KLH conjugated synthetic peptide derived from human AIF
Isotype IgG
Purification Purified by Protein A.
Target
Alternative Name AIF (AIFM1 Antibody Abstract)
Background

Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.

Subcellular location: Cytoplasm, Mitochondrion

Synonyms: AIF, CMT2D, CMTX4, COWCK, NADMR, NAMSD, PDCD8, COXPD6, Apoptosis-inducing factor 1, mitochondrial, Programmed cell death protein 8, AIFM1

Gene ID 9131
UniProt O95831
Pathways Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Images
Image no. 1 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 130-180) antibody (ABIN724010) Formalin-fixed and paraffin embedded labeled with human gastric carcinoma Rabbit Anti...
Image no. 2 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 130-180) antibody (ABIN724010) Formalin-fixed and paraffin embedded labeled with human gastric carcinoma Rabbit Anti...
Image no. 3 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 130-180) antibody (ABIN724010) Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AIF Polyclona...
Image no. 4 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 130-180) antibody (ABIN724010) Formalin-fixed and paraffin embedded human cervical tissue labeled with Anti-AIF Poly...
Image no. 5 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 130-180) antibody (ABIN724010) Mouse Liver lysates probed with AIF Polyclonal Antibody, unconjugated at 1:300 overn...
Image no. 6 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 130-180) antibody (ABIN724010) K562 lysates probed with AIF Polyclonal Antibody, unconjugated at 1:300 overnight at...
Image no. 7 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 130-180) antibody (ABIN724010) HT1080 lysates probed with Anti-AIF Polyclonal Antibody at 1:5000 for 90 min at 37˚C.
Image no. 8 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 130-180) antibody (ABIN724010) Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AIF Polyclona...
Image no. 9 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 130-180) antibody (ABIN724010) Formalin-fixed and paraffin embedded labeled with human gastric carcinoma Rabbit Anti...
Image no. 10 for anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 130-180) antibody (ABIN724010) Formalin-fixed and paraffin embedded labeled with human gastric carcinoma Rabbit Anti...
Product cited in: Li, Chen, Lu, Zhang, Guan, Li, Wang: "Anti-Oxidative Stress Activity Is Essential for Amanita caesarea Mediated Neuroprotection on Glutamate-Induced Apoptotic HT22 Cells and an Alzheimer's Disease Mouse Model." in: International journal of molecular sciences, Vol. 18, Issue 8, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Gong, Hua, Zhang, Zhao, Tang, Mei, Zhang, Cui, Li: "Hypothermia-induced neuroprotection is associated with reduced mitochondrial membrane permeability in a swine model of cardiac arrest." in: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, Vol. 33, Issue 6, pp. 928-34, 2013 (PubMed). Method employed by authors: Western Blotting (WB) (1:400, Sample species: Pig (Porcine)). Further details: Sample: frontal cortex