ACSS2 antibody (N-Term)

Catalog No. ABIN7565732

The Rabbit Polyclonal anti-ACSS2 antibody has been validated for WB and ELISA. It is suitable to detect ACSS2 in samples from Human.

Quick Overview for ACSS2 antibody (N-Term) (ABIN7565732)

Target: ACSS2 (Acyl-CoA Synthetase Short-Chain Family Member 2 (ACSS2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACSS2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ACSS2 Antibody

Binding Specificity: N-Term

Purpose: ACSS2 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human ACSS2 protein.

Characteristics: Synonyms: rabbit anti-ACSS2 antibody, ACSS-2, ACSS 2, Acetyl-coenzyme A synthetase (cytoplasmic), Acetate-CoA ligase, Acetyl-CoA synthetase, ACS, AceCS, Acyl-CoA synthetase short-chain family member 2, Acyl-activating enzyme, ACAS2

Purification: Anti-ACSS2 Antibody to was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: ACSS2 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic peptide near the N-terminus of human ACSS2.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes: Application Note: Anti-ACSS2 Antibody has been tested in Western Blot and ELISA. Expect a band at approximately 76 kDa in western blot using appropriate lysates. Positive control whole cell lysates used Hep-G2 at 1 μg/mL for WB. Although not tested, this antibody is likely functional in immunohistochemistry, immunofluorescence, and immunoprecipitation. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 1:10,000 - 1:50,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.06 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for ACSS2

Target: ACSS2 (Acyl-CoA Synthetase Short-Chain Family Member 2 (ACSS2))

Alternative Name: ACSS2

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) (NCI). ACSS2 (Acyl-CoA synthetase short-chain family member 2) is a protein coding gene encoding a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. This protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. This protein is upregulated in many cancers and is important for pro-survival under hypoxic conditions. ACSS2 may be associated with disorders such as Cystoisosporiasis. Anti-ACSS2 Antibody is useful for researchers interested in parasites, intestines, Glucose Energy Metabolism, and AKT research.

Gene ID: 55902

NCBI Accession: NP_001070020

UniProt: Q9NR19