Avidin antibody (FITC)
-
- Target See all Avidin (AVD) Antibodies
- Avidin (AVD)
-
Reactivity
- Chicken
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This Avidin antibody is conjugated to FITC
-
Application
- Western Blotting (WB), Dot Blot (DB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Purpose
- Avidin Antibody Fluorescein Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and Avidin.
- Characteristics
- Synonyms: rabbit anti-Avidin Antibody fluorescein Conjugation, FITC conjugated rabbit anti-Avidin antibody, Avidin fluorescein, Anti-Avidin FITC Antibody, egg ehite
- Purification
- Anti-Avidin Antibody Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Sterility
- Sterile filtered
- Immunogen
-
Immunogen: Avidin (Hen Egg White)
Immunogen Type: Native Protein
- Labeling Ratio
- 3.0
- Top Product
- Discover our top product AVD Primary Antibody
-
-
- Application Notes
- Application Note: Rabbit Anti-Avidin Fluorescein conjugate has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: User Optimized Western Blot Dilution: >1:1,000 FLISA Dilution: >1:1,000 IF Microscopy Dilution: User Optimized Other: User Optimized
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
-
- Target
- Avidin (AVD)
- Alternative Name
- AVIDIN (AVD Products)
- Background
- Background: Avidin is a glycoprotein with a molecular weight of approximately 62.4 kDa. Avidin is a biotin binding protein that shows high sequence homology in birds, reptiles and amphibians. Hen egg white avidin is a tetrameric protein composed of four identical subunits, each with the ability to bind biotin with high affinity and specificity (Kd ~ 1015 M). In biotechnology, the functional consequence of tetrameric biotin binding is signal amplification. Biotin-avidin bridging is a great way to increase signal strength while maintaining specificity. The sequence of avidin only shows 30 % homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive.
- UniProt
- P02701
-