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Avidin antibody (FITC)

AVD Reactivity: Chicken WB, DB, FACS, FLISA, FM Host: Rabbit Polyclonal FITC
Catalog No. ABIN7565743
  • Target See all Avidin (AVD) Antibodies
    Avidin (AVD)
    Reactivity
    • 12
    • 7
    • 7
    • 1
    Chicken
    Host
    • 16
    • 8
    • 1
    • 1
    Rabbit
    Clonality
    • 18
    • 8
    Polyclonal
    Conjugate
    • 6
    • 5
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    This Avidin antibody is conjugated to FITC
    Application
    • 21
    • 19
    • 17
    • 7
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Dot Blot (DB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Purpose
    Avidin Antibody Fluorescein Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and Avidin.
    Characteristics
    Synonyms: rabbit anti-Avidin Antibody fluorescein Conjugation, FITC conjugated rabbit anti-Avidin antibody, Avidin fluorescein, Anti-Avidin FITC Antibody, egg ehite
    Purification
    Anti-Avidin Antibody Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Avidin (Hen Egg White)

    Immunogen Type: Native Protein

    Labeling Ratio
    3.0
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  • Application Notes
    Application Note: Rabbit Anti-Avidin Fluorescein conjugate has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: User Optimized Western Blot Dilution: >1:1,000 FLISA Dilution: >1:1,000 IF Microscopy Dilution: User Optimized Other: User Optimized
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Expiry Date
    12 months
  • Target
    Avidin (AVD)
    Alternative Name
    AVIDIN (AVD Products)
    Background
    Background: Avidin is a glycoprotein with a molecular weight of approximately 62.4 kDa. Avidin is a biotin binding protein that shows high sequence homology in birds, reptiles and amphibians. Hen egg white avidin is a tetrameric protein composed of four identical subunits, each with the ability to bind biotin with high affinity and specificity (Kd ~ 1015 M). In biotechnology, the functional consequence of tetrameric biotin binding is signal amplification. Biotin-avidin bridging is a great way to increase signal strength while maintaining specificity. The sequence of avidin only shows 30 % homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive.
    UniProt
    P02701
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