HbBs (N-Term) antibody

Catalog No. ABIN7565773

This Mouse Monoclonal antibody specifically detects in ELISA and WB. It exhibits reactivity toward Human.

Quick Overview for HbBs (N-Term) antibody (ABIN7565773)

Target: HbBs

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Clone: 23E5-H6-G6-C1-H7-F7-G9-F6

Product Details

Binding Specificity: N-Term

Purpose: Hemoglobin beta S Antibody

Cross-Reactivity (Details): This protein A purified mouse monoclonal antibody reacts specifically with human HbS beta sickle isoform.

Characteristics: Synonyms: mouse anti-HbS antibody, mouse anti-hemoglobin antibody, Hemoglobin beta subunit sickle mutant, HBS, HBBs, HbS Antibody, Sickle Cell Disease (SCD)

Purification: Anti-HbS is purified from tissue culture supernatant by protein A purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Hemoglobin beta S Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb beta-subunit conjugated to KLH.

Immunogen Type: Conjugated Peptide

Isotype: IgG3 kappa

Application Details

Application Notes: Application Note: Anti-Hemoglobin beta S (MOUSE) antibody has been tested by ELISA and western blot. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa in appropriate lysates. Western Blot Dilution: 1 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details

Target: HbBs

Background: Background: HbS antibodies detect the E6V mutant in the hemoglobin beta subunit. Functional adult hemoglobin (Hb) is a hetero tetramer composed of 2 alpha and 2 beta subunits (α2β2). Common isoform variants of hemoglobin include HbA, HbS, HbC, HbF, and HbA2. Hemoglobin S is the predominant hemoglobin in people with sickle cell disease. The alpha chain is normal. The disease-producing mutation exists in the beta chain, giving the molecule the structure, α2βS2. People who have one sickle mutant gene and one normal beta gene have sickle cell trait which is benign. Globin gene mutations affect the structure and expression levels of Hb. Sickle cell disease and the more benign sickle cell trait are observed in more than 100 million people globally. Perhaps the most significant mutation is the E6V in the beta subunit and the cause of SCD, but other relevant isoforms of Hb are observed. HbS antibody does not react to other forms of Hb. This antibody is ideal for investigators involved in Cardiovascular and developmental biology research.

UniProt: P68871