Hemoglobin Subunit beta antibody (N-Term)

Catalog No. ABIN7565774

The Mouse Monoclonal anti-Hemoglobin Subunit beta antibody has been validated for WB and ELISA. It is suitable to detect Hemoglobin Subunit beta in samples from Human.

Quick Overview for Hemoglobin Subunit beta antibody (N-Term) (ABIN7565774)

Target: Hemoglobin Subunit beta (HBB) (Hemoglobin beta (HBB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hemoglobin Subunit beta antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 14G2-G11-F11

Product Details anti-Hemoglobin Subunit beta Antibody

Binding Specificity: N-Term

Purpose: Hemoglobin A (beta chain) Antibody

Cross-Reactivity (Details): This protein A purified mouse monoclonal antibody reacts specifically with human HbA beta chain isoform.

Characteristics: Synonyms: mouse anti-HbA antibody, mouse anti-hemoglobin antibody, Hemoglobin beta subunit, Hemoglobin subunit beta, Hemoglobin beta chain, HBB, HBA, Hbβ Antibody, LVV-hemorphin-7, Spinorphin, Sickle Cell Disease (SCD)

Purification: Anti-Hemoglobin beta β is purified from tissue culture supernatant by protein A purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Hemoglobin A (beta chain) Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb beta-subunit conjugated to KLH.

Immunogen Type: Conjugated Peptide

Isotype: IgG2a lambda

Application Details

Application Notes: Application Note: Anti-Hemoglobin A (beta chain) (MOUSE) antibody has been tested by ELISA and western blot. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa. Western Blot Dilution: 1 μg/mL ELISA Dilution: 1:20,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.00 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Hemoglobin Subunit beta

Target: Hemoglobin Subunit beta (HBB) (Hemoglobin beta (HBB))

Alternative Name: HBB

Background: Background: HbA antibodies detect the hemoglobin beta subunit wild type variant A isoform. Functional adult hemoglobin (Hb) is a hetero tetramer composed of 2 alpha and 2 beta subunits (α2β2). Common isoform variants of hemoglobin include HbA, HbS, HbC, HbF, and HbA-2. Sickle cell disease (SCD), thalassemias and hemoglobinopathies occur when aberrant forms of hemoglobin are expressed in children and adults. Globin gene mutations affect the structure and expression levels of Hb. Sickle cell disease and the more benign sickle cell trait are observed in more than 100 million people globally. Perhaps the most significant mutation is the E6V in the beta subunit and the cause of SCD, but other relevant isoforms of Hb are observed. HbA antibody cross reacts with HbA-2 but does not react other forms Hb. This antibody is ideal for investigators involved in Cardiovascular and developmental biology research.

Gene ID: 3043

NCBI Accession: NP_000509

UniProt: P68871