Lactate Dehydrogenase A antibody (HRP)

Catalog No. ABIN7565783

The Goat Polyclonal anti-Lactate Dehydrogenase A antibody has been validated for WB, IHC, ELISA, IP and IF. It is suitable to detect Lactate Dehydrogenase A in samples from Rabbit.

Quick Overview for Lactate Dehydrogenase A antibody (HRP) (ABIN7565783)

Target: Lactate Dehydrogenase A (LDHA)

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This Lactate Dehydrogenase A antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-Lactate Dehydrogenase A Antibody (HRP)

Purpose: Lactate Dehydrogenase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].

Characteristics: Synonyms: goat anti-Lactate Dehydrogenase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Lactate Dehydrogenase Antibody, L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M

Purification: Anti-Lactate Dehydrogenase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Lactate Dehydrogenase Antibody was produced by repeated immunizations with rabbit muscle Lactate Dehydrogenase full length protein.

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Lactate Dehydrogenase peroxidase Antibody has been tested in ELISA, western blot, and immunofluorescence. This product is suitable for IP and IHC. Researchers should determine optimal titers for applications that are not stated below. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:6,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:3,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Lactate Dehydrogenase A

Target: Lactate Dehydrogenase A (LDHA)

Alternative Name: LDHA

Background: Background: Anti-Lactate Dehydrogenase Antibody detects Lactate Dehydrogenase. Lactate dehydrogenases exist in four distinct enzyme classes. Two of them are cytochrome c-dependent enzymes, each acting on either D-lactate or L-lactate. The other two are NAD(P)-dependent enzymes, each acting on either D-lactate or L-lactate. Lactate dehydrogenase catalyzes the interconversion of pyruvate and lactate. Anti-Lactate Dehydrogenase Antibody is ideal for investigators involved in Cell Signaling, Immunology, Neuroscience and Signal Transduction research.

Gene ID: 100009107, 126050

UniProt: P13491

Pathways: Warburg Effect