Lactate Dehydrogenase A antibody (Middle Region)

Catalog No. ABIN2785876

This Rabbit Polyclonal antibody specifically detects Lactate Dehydrogenase A in WB, IHC and IP. It exhibits reactivity toward Human, Mouse, Rat, Rabbit, Cow, Horse, Dog, Guinea Pig, Goat and Zebrafish (Danio rerio).It has been mentioned in 1 publication

Quick Overview for Lactate Dehydrogenase A antibody (Middle Region) (ABIN2785876)

Target: Lactate Dehydrogenase A (LDHA)

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Horse, Dog, Guinea Pig, Goat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lactate Dehydrogenase A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-Lactate Dehydrogenase A Antibody

Binding Specificity: Middle Region

Purpose: LDHA Antibody - middle region

Sequence: PLSCHGWVLG EHGDSSVPVW SGMNVAGVSL KTLHPDLGTD KDKEQWKEVH

Predicted Reactivity: Cow: 79%, Dog: 86%, Goat: 79%, Guinea Pig: 79%, Horse: 79%, Human: 100%, Mouse: 86%, Rabbit: 86%, Rat: 79%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against LDHA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human LDHA

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54777-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Lactate Dehydrogenase A antibody (ABIN2785876)

Listerman, Bledau, Grishina, Neugebauer: "Extragenic accumulation of RNA polymerase II enhances transcription by RNA polymerase III." in: PLoS genetics, Vol. 3, Issue 11, pp. e212, (2008) (PubMed).

Target Details for Lactate Dehydrogenase A

Target: Lactate Dehydrogenase A (LDHA)

Alternative Name: LDHA

Background:

Background Information: The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.

Gene Name: lactate dehydrogenase A

Alternative Symbols: LDHM, GSD11, PIG19, HEL-S-133P

Protein Name: L-lactate dehydrogenase A chain

Molecular Weight: 37kDa

Gene ID: 3939

NCBI Accession: NP_005557

UniProt: P00338

Pathways: Warburg Effect