PAX7 antibody (Internal Region)

Catalog No. ABIN7565803

This Rabbit Polyclonal antibody specifically detects PAX7 in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for PAX7 antibody (Internal Region) (ABIN7565803)

Target: PAX7 (Paired Box 7 (PAX7))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAX7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PAX7 Antibody

Binding Specificity: Internal Region

Purpose: Pax7 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross reactivity with Pax7 protein from mouse (100 % homology) and human (66.

Characteristics: Synonyms: rabbit anti-Pax7 antibody, Paired box protein Pax-7, Pax 7, Pax-7

Purification: Anti-Pax-7 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Pax7 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Pax7 protein.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes: Application Note: Anti-Pax7 antibody has been tested in ELISA and Western Blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 54.9 kDa in size corresponding to Pax7 by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 1:10,000-1:40:000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for PAX7

Target: PAX7 (Paired Box 7 (PAX7))

Alternative Name: Pax7

Background: Background: The Anti-Pax7 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Paired box protein Pax-7 transcription factor plays a role in myogenesis through regulation of muscle precursor cells proliferation. These genes play critical roles during fetal development and cancer growth. It is expressed specifically during the development of the nervous and muscular system. Anti-Pax7 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.

Gene ID: 18509

NCBI Accession: NP_035169

UniProt: P47239