Protein A antibody (Biotin)

Catalog No. ABIN7565826

This anti-Protein A antibody is a Goat Polyclonal antibody detecting Protein A in ELISA, WB, IHC and DB. Suitable for Staphylococcus aureus.

Quick Overview for Protein A antibody (Biotin) (ABIN7565826)

Target: Protein A

Reactivity: Staphylococcus aureus

Host: Goat

Clonality: Polyclonal

Conjugate: This Protein A antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)

Product Details anti-Protein A Antibody (Biotin)

Purpose: Protein A Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Protein A [Staphylococcus aureus].

Characteristics: Synonyms: goat anti-Protein A Antibody, Biotin Conjugated goat anti-Protein A Antibody, Protein A Biotin, Protein A BAC Antibody

Purification: Anti-Protein A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Protein A [Staphylococcus aureus]

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Protein A Biotin Conjugated has been tested by dot blot and western blot. This product is suitable to be assayed against 1.0 μg of Protein A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:500-1:2,500 Western Blot Dilution: 1:1,000-1:5,000 ELISA Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Protein A

Target: Protein A

Background: Background: Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Biotinylated proteins of interest can be enriched from a sample due to highly stable interactions. Biotin conjugated anti-Protein A antibodies are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications.