Protein A antibody (Texas Red (TR))

Catalog No. ABIN7565827

This Goat Polyclonal antibody specifically detects Protein A in FACS and FM. It exhibits reactivity toward Staphylococcus aureus.

Quick Overview for Protein A antibody (Texas Red (TR)) (ABIN7565827)

Target: Protein A

Reactivity: Staphylococcus aureus

Host: Goat

Clonality: Polyclonal

Conjugate: This Protein A antibody is conjugated to Texas Red (TR)

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Product Details anti-Protein A Antibody (Texas Red (TR))

Purpose: Protein A Antibody Texas Red™ Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus], anti-Goat IgG and anti-Goat Serum.

Characteristics: Synonyms: goat anti-Protein A Antibody, Texas Red™ Conjugated goat anti-Protein A Antibody, Protein A Texas Red™, Texas Red™, Protein A Texas Red™ Antibody

Purification: Anti-Protein A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Protein A [Staphylococcus aureus]

Immunogen Type: Native Protein

Labeling Ratio: 1.89

Application Details

Application Notes: Application Note: Anti-Protein A Texas Red Conjugated Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Flow Cytometry Dilution: User Optimized IF Microscopy Dilution: 1:500 - 1:2,500 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Protein A

Target: Protein A

Background: Background: Protein A is 56 kDA surface protein found in the cell wall of Staphylococcus aureus. Protein A has a high affinity to Human IgG, Mouse IgG and others and is therefore suitable for purification and detection of immunoglobulins.