RFP antibody

Catalog No. ABIN7565836

This Mouse Monoclonal antibody specifically detects RFP in WB, ELISA, IP, FACS and FM. It exhibits reactivity toward Discosoma.

Quick Overview for RFP antibody (ABIN7565836)

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma

Host: Mouse

Clonality: Monoclonal

Conjugate: This RFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Clone: 8E5-G7

Product Details anti-RFP Antibody

Purpose: RFP Antibody

Cross-Reactivity (Details): Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.

Characteristics: Synonyms: mouse anti-RFP Antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

Purification: Anti-RFP Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.

Immunogen Type: Recombinant Protein

Isotype: IgG2a kappa

Application Details

Application Notes: Application Note: Monoclonal anti-RFP is designed to detect RFP and its variants. This antibody has been tested by ELISA and Western blot, and is suitable for use in FISH, IF, IHC, and multiplex assays based on published references. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Optimal titers for applications should be determined by the researcher. Flow Cytometry Dilution: User Optimized Western Blot Dilution: 1:1,000 - 1:10,000 Immunoprecipitation Dilution: User Optimized ELISA Dilution: 1:75,000 - 1:150,000 IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store Anti-RFP at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: DsRed

Background: Background: Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, Western blotting, immunohistochemistry, and flow cytometry. RFP Proteins are useful markers for imagining protein localization, monitoring physiological processes, and detecting transgenic expression. Rockland's anti-RFP antibody can be used to detect native RFP and RFP variants.

UniProt: Q9U6Y8