Transferrin antibody
Quick Overview for Transferrin antibody (ABIN7565850)
Target
See all Transferrin (TF) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Purpose
- Transferrin Antibody
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Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Transferrin [Human Serum].
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Characteristics
- Synonyms: rabbit anti-Transferrin Antibody, Apotransferrin antibody, Beta 1 metal binding globulin antibody, DKFZp781D0156 antibody, PRO1400 antibody, PRO1557 antibody, PRO2086 antibody, Serotransferrin precursor antibody, Siderophilin antibody, TF antibody
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Purification
- Anti-Transferrin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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Sterility
- Sterile filtered
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Immunogen
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Immunogen: Transferrin Antibody was produced by repeated immunizations with human serum Transferrin protein.
Immunogen Type: Native Protein
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Application Notes
- Application Note: Anti-Transferrin Antibody has been tested in western blotting, ELISA and IHC. Researchers should determine optimal titers for applications that are not stated below. Immunohistochemistry Dilution: 1:500 - 1:10,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:150,000 Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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Expiry Date
- 12 months
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- Transferrin (TF)
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Alternative Name
- Transferrin
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Background
- Background: Transferrin Antibody detects transferrin protein. Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Anti-transferrin Antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.
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Gene ID
- 7018
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UniProt
- P02787
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Pathways
- Transition Metal Ion Homeostasis
Target
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