TUBA1B antibody (C-Term)

Catalog No. ABIN7565852

The Mouse Monoclonal anti-TUBA1B antibody has been validated for WB, ELISA, IF, IHC, FM and MA. It is suitable to detect TUBA1B in samples from Human.

Quick Overview for TUBA1B antibody (C-Term) (ABIN7565852)

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBA1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Clone: 17H11-F10

Product Details anti-TUBA1B Antibody

Binding Specificity: C-Term

Purpose: Alpha-Tubulin Antibody

Cross-Reactivity (Details): This Loading Control antibody is directed against alpha tubulin.

Characteristics: Synonyms: mouse anti-Alpha-Tubulin Antibody, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control

Purification: Anti-Tubulin Loading Control Antibody was purified by Protein A chromatography.

Sterility: Sterile filtered

Immunogen: Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.

Isotype: IgG1 kappa

Application Details

Application Notes: Application Note: Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts. Immunohistochemistry Dilution: 2.5 μg/mL Western Blot Dilution: 1:1,000 ELISA Dilution: 1:300,000 IF Microscopy Dilution: 0.1 μg/mL Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for TUBA1B

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Alternative Name: TUBA1B

Background: Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  This antibody makes an excellent loading control.

Gene ID: 17986283

UniProt: P68363

Pathways: Microtubule Dynamics, M Phase