CPA1 antibody (HRP)

Catalog No. ABIN7565867

The Rabbit Polyclonal anti-CPA1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect CPA1 in samples from Cow.

Quick Overview for CPA1 antibody (HRP) (ABIN7565867)

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPA1 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-CPA1 Antibody (HRP)

Purpose: Carboxypeptidase A Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase A [Bovine Pancreas].

Characteristics: Synonyms: rabbit anti-Carboxypeptidase A antibody peroxidase conjugation, HRP conjugated rabbit anti-Carboxypeptidase A Antibody, Carboxypeptidase A1 precursor antibody, CPA1 antibody, Pancreatic carboxypeptidase A1 antibody, Procarboxypeptidase A1 pancreatic antibody

Purification: Carboxypeptidase A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Carboxypeptidase A [Bovine Pancreas]

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Carboxypeptidase A Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is useful in immunohistochemistry. Optimal titers should be obtained by researchers. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:300 - 1:2,000 ELISA Dilution: 1:3,000 - 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CPA1

Target: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Alternative Name: CPA1

Background: Background: Carboxypeptidase A (CPA-1) is produced in the pancreas and is crucial to many processes in the human body to include digestion, post-translational modification of proteins, blood clotting, and reproduction. There are two proposed mechanisms for the catalytic function of carboxypeptidase A. The first is a nucleophilic pathway involving a covalent acyl enzyme intermediate containing active site base Glu-270. The second proposed mechanism is a promoted water pathway. This mechanism involves attack of a water molecule at the scissile peptide linkage of the substrate.

Gene ID: 286762

NCBI Accession: NP_777175

UniProt: P00730