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DNASE1 antibody (HRP)

This anti-DNASE1 antibody is a Rabbit Polyclonal antibody detecting DNASE1 in WB, ELISA and IP. Suitable for Cow.
Catalog No. ABIN7565874

Quick Overview for DNASE1 antibody (HRP) (ABIN7565874)

Target

See all DNASE1 Antibodies
DNASE1 (Deoxyribonuclease I (DNASE1))

Reactivity

  • 70
  • 26
  • 16
  • 16
Cow

Host

  • 68
  • 17
  • 1
Rabbit

Clonality

  • 71
  • 15
Polyclonal

Conjugate

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  • 9
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This DNASE1 antibody is conjugated to HRP

Application

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  • 15
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Western Blotting (WB), ELISA, Immunoprecipitation (IP)
  • Purpose

    Deoxyribonuclease I Antibody Peroxidase Conjugated

    Cross-Reactivity (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Deoxyribonuclease I [Bovine Pancreas].

    Characteristics

    Synonyms: rabbit anti-Deoxyribonuclease I Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Deoxyribonuclease I Antibody, Deoxyribonuclease-1, Deoxyribonuclease I, DNase I

    Purification

    Deoxyribonuclease I is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    Sterility

    Sterile filtered

    Immunogen

    Immunogen: Deoxyribonuclease I [Bovine Pancreas]

    Immunogen Type: Native Protein

  • Application Notes

    Application Note: Anti-Deoxyribonuclease I Peroxidase Conjugated Antibody has been tested by ELISA and Western blot. This product is assayed against 1.0 μg of Deoxyribonuclease I [Bovine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:5,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

    Expiry Date

    12 months
  • Target

    DNASE1 (Deoxyribonuclease I (DNASE1))

    Alternative Name

    DNASE1, DNL1

    Background

    Background: Deoxyribonuclease I (DNase I) is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin.

    Gene ID

    282217

    NCBI Accession

    NP_776959

    UniProt

    P00639
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