KCTD8 antibody (HRP)

Catalog No. ABIN7565875

This Rabbit Polyclonal antibody specifically detects KCTD8 in WB, ELISA and IP. It exhibits reactivity toward Penicillium sp..

Quick Overview for KCTD8 antibody (HRP) (ABIN7565875)

Target: KCTD8 (Potassium Channel Tetramerisation Domain Containing 8 (KCTD8))

Reactivity: Penicillium sp.

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCTD8 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-KCTD8 Antibody (HRP)

Purpose: Dextranase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Dextranase [Penicillium species].

Characteristics: Synonyms: rabbit anti-Dextranase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Dextranase Antibody, dextranase, Alpha-1,6-glucan-6-glucanohydrolase, DEX

Purification: Dextranase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Dextranase [Penicillium species]

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Dextranase Peroxidase has been tested by ELISA and western blot and assayed against 1.0 μg of Dextranase [Penicillium species] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:5,000 Immunoprecipitation Dilution: User Optimized ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for KCTD8

Target: KCTD8 (Potassium Channel Tetramerisation Domain Containing 8 (KCTD8))

Alternative Name: DEX

Background: Background: Dextranase belongs to the glycosyl hydrolase 49 family. It's catalytic reaction is endohydrolysis of (1->6)-alpha-D-glucosidic linkages in dextran.

UniProt: P48845

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling