CES3 antibody (HRP)

Catalog No. ABIN7565877

This anti-CES3 antibody is a Rabbit Polyclonal antibody detecting CES3 in WB, ELISA and IHC. Suitable for Pig.

Quick Overview for CES3 antibody (HRP) (ABIN7565877)

Target: CES3 (Carboxylesterase 3 (CES3))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CES3 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-CES3 Antibody (HRP)

Purpose: Esterase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Esterase [Porcine Liver].

Characteristics: Synonyms: rabbit anti-Esterase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Esterase Antibody, Liver carboxylesterase, Proline-beta-naphthylamidase, Retinyl ester hydrolase

Purification: Esterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Esterase [Porcine Liver]

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Esterase Peroxidase conjugated antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Esterase [Porcine Liver] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,500 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:250 - 1:1,000 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CES3

Target: CES3 (Carboxylesterase 3 (CES3))

Alternative Name: CES3

Background: Background: Esterase is a hydrolase enzyme that splits esters into an acid and an alcohol in a chemical reaction with water called hydrolysis.

Gene ID: 100626873, 343791015

UniProt: Q29550

Pathways: Monocarboxylic Acid Catabolic Process