LPO antibody (HRP)

Catalog No. ABIN7565890

This Sheep Polyclonal antibody specifically detects LPO in WB, ELISA, IP and DB. It exhibits reactivity toward Cow.

Quick Overview for LPO antibody (HRP) (ABIN7565890)

Target: LPO

Reactivity: Cow

Host: Sheep

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Product Details

Purpose: Lactoperoxidase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Lactoperoxidase [Bovine Milk].

Characteristics: Synonyms: sheep anti-Lactoperoxidase HRP conjugated Antibody, peroxidase conjugated sheep anti-Lactoperoxidase Antibody, LPO antibody, Salivary peroxidase antibody, SAPX antibody, SPO antibody

Purification: Lactoperoxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Lactoperoxidase [Bovine Milk]

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Lactoperoxidase Peroxidase Conjugated Antibody has been tested by dot blot and western blot and is suitable to be assayed against 1.0 μg of Lactoperoxidase [Bovine Milk] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for Anti-Lactoperoxidase Antibody. Western Blot Dilution: 1:500 - 1:5,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details

Target: LPO

Background: Background: Lactoperoxidase antibody recognizes the lactoperoxidase protein. Lactoperoxidase catalyzes the oxidation of a number of inorganic and organic substrates by hydrogen peroxide. Lactoperoxidase plays an important role in killing bacteria in milk. Lactoperoxidase conjugated to peroxidase is used to bind to the heavy and light specific target of the lactoperoxidase protein. Lactoperoxidase conjugated to Peroxidase is suitable for researchers in immunology, cancer, and biochemistry.

Gene ID: 280844

UniProt: P80025