GALM antibody (HRP)

Catalog No. ABIN7565898

This anti-GALM antibody is a Rabbit Polyclonal antibody detecting GALM in WB, ELISA and IP. Suitable for Pig.

Quick Overview for GALM antibody (HRP) (ABIN7565898)

Target: GALM (Galactose Mutarotase (Aldose 1-Epimerase) (GALM))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GALM antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-GALM Antibody (HRP)

Purpose: Mutarotase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Mutarotase [Porcine Kidney].

Characteristics: Synonyms: rabbit anti-Mutarotase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Mutarotase Antibody, Aldose 1 epimerase antibody, BLOCK25 protein antibody, Galactose mutarotase antibody, GALM antibody

Purification: Mutarotase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Mutarotase [Porcine Kidney]

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Mutarotase Peroxidase Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Mutarotase [Porcine Kidney] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:1,500 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:5,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for GALM

Target: GALM (Galactose Mutarotase (Aldose 1-Epimerase) (GALM))

Alternative Name: GALM

Background: Background: Mutarotase is found especially in mammalian tissues that catalyzes the interconversion of anomeric forms of some sugars: α- and β- forms of d-glucose, l-arabinose, d-galactose, d-xylose, lactose, and maltose.

Gene ID: 399536

UniProt: Q9GKX6