PGC antibody (HRP)

Catalog No. ABIN7565906

The Rabbit Polyclonal anti-PGC antibody has been validated for WB, ELISA and IHC. It is suitable to detect PGC in samples from Zea mays.

Quick Overview for PGC antibody (HRP) (ABIN7565906)

Target: PGC (Pepsinogen C (PGC))

Reactivity: Zea mays

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PGC antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-PGC Antibody (HRP)

Purpose: Phospho Enol Pyruvate Carboxylase Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Phospho-enol-Pyruvate Carboxylase [Maize].

Characteristics: Synonyms: rabbit anti-Phospho Enol Pyruvate Carboxylase Antibody Peroxidase Conjugation, PE conjugated rabbit anti-PEPC 1 antibody, PEPCase 1 antibody, Phosphoenolpyruvate carboxylase 1 antibody

Purification: Phospho Enol Pyruvate Carboxylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Phospho-enol-pyruvate Carboxylase [Maize Leaves]

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Phosphoenolpyruvate Carboxylase Peroxidase conjugated antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Phospho-enol-Pyruvate Carboxylase [Maize] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for PGC

Target: PGC (Pepsinogen C (PGC))

Alternative Name: pepc

Background: Background: Phospho Enol Pyruvate Carboxylase is an enzyme in the family of carboxy-lyases that catalyzes the addition of bicarbonate to phosphoenolpyruvate (PEP) to form a four-carbon compound oxaloacetate. This reaction is used for carbon fixation in CAM and C4 plants where it plays a key role in photosynthesis. The enzyme is also found in some bacteria, but not in animals or fungi.

Gene ID: 542372

UniProt: B8XPZ2