Streptavidin antibody (Texas Red (TR))

Catalog No. ABIN7565913

This anti-Streptavidin antibody is a Rabbit Polyclonal antibody detecting Streptavidin in WB, FACS, DB, FM and FLISA. Suitable for Streptomyces avidinii.

Quick Overview for Streptavidin antibody (Texas Red (TR)) (ABIN7565913)

Target: Streptavidin

Reactivity: Streptomyces avidinii

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Streptavidin antibody is conjugated to Texas Red (TR)

Application: Western Blotting (WB), Flow Cytometry (FACS), Dot Blot (DB), Fluorescence Microscopy (FM), FLISA

Product Details anti-Streptavidin Antibody (Texas Red (TR))

Purpose: Streptavidin Antibody Texas Red™ Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Streptavidin.

Characteristics: Synonyms: rabbit anti-Streptavidin Antibody Texas Red™ Conjugation, Texas Red™ conjugated rabbit anti-Streptavidin antibody, Anti-Streptavidin Texas Red™ Antibody

Purification: Streptavidin Antibody Texas Red™ Conjugated was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Streptavidin (Streptomyces avidinii)

Immunogen Type: Native Protein

Labeling Ratio: 1.4

Application Details

Application Notes: Application Note: Streptavidin Antibody Texas Red™ Conjugated antibody has been tested by dot blot and western blot and are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: User Optimized FLISA Dilution: User Optimized IF Microscopy Dilution: 1:100-1:500 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Streptavidin

Target: Streptavidin

Background: Background: STREPTAVIDIN antibody is a 60 kDa protein purified from the bacterium Streptomyces avidinii. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin (also known as vitamin B7). With a dissociation constant (Kd) on the order of ~10-14 Mol/L,[1] the binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Streptavidin is used extensively in molecular biology and bio-nanotechnology due to the streptavidin-biotin complex's resistance to organic solvents, denaturants (e.g. guanidinium chloride), detergents (e.g. SDS, Triton), proteolytic enzymes, and extremes of temperature and pH . Anti-STREPTAVIDIN antibody is ideal for investigators involved in GFP and Epitope research.