ORAI1 antibody

Catalog No. ABIN7565939

The Rabbit Polyclonal anti-ORAI1 antibody has been validated for WB. It is suitable to detect ORAI1 in samples from Human.

Quick Overview for ORAI1 antibody (ABIN7565939)

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ORAI1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ORAI1 Antibody

Purpose: ORAI1 Antibody

Cross-Reactivity (Details): Anti-ORAI-1 antibody is directed against the human ORAI-1.

Characteristics: Synonyms: rabbit anti-ORAI-1 Antibody, Calcium release-activated calcium channel protein 1, orai-1, Transmembrane protein 142A, ORAI1, CRACM1, TMEM142A

Purification: The product was prepared from monospecific antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen:

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a recombinant fusion protein.

Immunogen Type: Recombinant Protein

Application Details

Application Notes: Application Note: Anti-ORAI-1 has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect bands approximately 32.7 kDa by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 45 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for ORAI1

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Alternative Name: ORAI1

Background: Background: The ORAI-1 gene codes for the calcium release-activated calcium (CRAC) channel subunit in humans. The CRAC channel subunit mediates calcium influx when activated by the calcium sensor, STIM1, following depletion of intracellular calcium stores. CRAC channels are the primary pathway for calcium influx in T-cells and promote the immune response to pathogens through activation of the transcription factor NFAT. Defects in this gene can be a cause of immune dysfunction due to inactivation of T-cells.

Gene ID: 84876

NCBI Accession: NP_116179

UniProt: Q96D31

Pathways: TCR Signaling, BCR Signaling