HIST1H3A antibody (C-Term)

Catalog No. ABIN7565940

This anti-HIST1H3A antibody is a Rabbit Polyclonal antibody detecting HIST1H3A in ELISA, WB and IHC. Suitable for Human.

Quick Overview for HIST1H3A antibody (C-Term) (ABIN7565940)

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST1H3A antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HIST1H3A Antibody

Binding Specificity: C-Term

Purpose: Histone H3 Antibody

Cross-Reactivity (Details): Anti-Histone H3 is directed against the human histone 3 protein.

Characteristics: Synonyms: rabbit anti-histone H3 antibody, Histone H3.1, H3F, H3FA, H3FB, H3FL, H3FL, H3FC, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ, Histone 3 Antibody

Purification: The product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Histone-3 was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to the c-terminus region of human histone-3.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes: Application Note: Histone H3 antibody has been tested for use in ELISA and western blot. This antibody is suitable for IHC. For western blots expect a band of approximately 15.4 kDa in size corresponding to the Histone 3 protein. Specific conditions for reactivity should be optimized by the end user. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 5.0 μg/mL Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.98 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store H3 antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for HIST1H3A

Target: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Alternative Name: HIST1H3A

Background: Background: Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone proteins are highly post-translationally modified with Histone H3 being the most extensively modified of the five histones. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 Antibody is ideal for investigators involved in Cell Signaling, Epigenetics, Nuclear Signaling research and Signal Transduction research.

Gene ID: 8350

NCBI Accession: NP_003520

UniProt: P68431