CH25H antibody (C-Term)

Catalog No. ABIN7565948

The Rabbit Polyclonal anti-CH25H antibody has been validated for WB, ELISA and IHC. It is suitable to detect CH25H in samples from Human.

Quick Overview for CH25H antibody (C-Term) (ABIN7565948)

Target: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CH25H antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-CH25H Antibody

Binding Specificity: C-Term

Purpose: CH25H Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human CH25H.

Characteristics: Synonyms: Rabbit Anti-Cholesterol 25-Hydroxylase, Rabbit Anti-CH25H, Cholesterol 25-Monooxygenase, EC 1.14.99.38, H25OH, C25H

Purification: This affinity purified antibody is directed against human CH25H.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-CH25H antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of human CH25H conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Application Details

Application Notes: Application Note: Anti-CH25H Antibody has been tested in ELISA, WB, and IHC.  Expect a band at ~31.7 kDa in western blot using appropriate tissues or lysates.  Positive control used: Human Kidney Tissues in Immunohistochemistry. Immunohistochemistry Dilution: 1:100 Western Blot Dilution: 1:1,000 ELISA Dilution: 1:10,000 - 1:50,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.92 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CH25H

Target: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Alternative Name: CH25H

Background: Background: CH25H (Cholesterol 25-Hydroxylase catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. CH25H plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for several lymphoid cells. It may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. Anti-CH25H Antibody is useful for researchers interested in Alzheimer's Disease, Neuroscience, and metabolism.

Gene ID: 9023

NCBI Accession: NP_003947

UniProt: O95992