CH25H antibody (C-Term)
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- Target See all CH25H Antibodies
- CH25H (Cholesterol 25-Hydroxylase (CH25H))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CH25H antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Purpose
- CH25H Antibody
- Cross-Reactivity (Details)
- This affinity purified antibody is directed against human CH25H.
- Characteristics
- Synonyms: Rabbit Anti-Cholesterol 25-Hydroxylase, Rabbit Anti-CH25H, Cholesterol 25-Monooxygenase, EC 1.14.99.38, H25OH, C25H
- Purification
- This affinity purified antibody is directed against human CH25H.
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: Anti-CH25H antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of human CH25H conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Conjugated Peptide
- Top Product
- Discover our top product CH25H Primary Antibody
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- Application Notes
- Application Note: Anti-CH25H Antibody has been tested in ELISA, WB, and IHC. Expect a band at ~31.7 kDa in western blot using appropriate tissues or lysates. Positive control used: Human Kidney Tissues in Immunohistochemistry. Immunohistochemistry Dilution: 1:100 Western Blot Dilution: 1:1,000 ELISA Dilution: 1:10,000 - 1:50,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.92 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- CH25H (Cholesterol 25-Hydroxylase (CH25H))
- Alternative Name
- CH25H (CH25H Products)
- Background
- Background: CH25H (Cholesterol 25-Hydroxylase catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. CH25H plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for several lymphoid cells. It may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. Anti-CH25H Antibody is useful for researchers interested in Alzheimer's Disease, Neuroscience, and metabolism.
- Gene ID
- 9023
- NCBI Accession
- NP_003947
- UniProt
- O95992
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