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CH25H antibody (C-Term)

CH25H Reactivity: Human ELISA, WB, IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7565948
  • Target See all CH25H Antibodies
    CH25H (Cholesterol 25-Hydroxylase (CH25H))
    Binding Specificity
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 13
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 11
    • 3
    Rabbit
    Clonality
    • 12
    • 2
    Polyclonal
    Conjugate
    • 10
    • 2
    • 1
    • 1
    This CH25H antibody is un-conjugated
    Application
    • 9
    • 7
    • 5
    • 2
    • 2
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
    Purpose
    CH25H Antibody
    Cross-Reactivity (Details)
    This affinity purified antibody is directed against human CH25H.
    Characteristics
    Synonyms: Rabbit Anti-Cholesterol 25-Hydroxylase, Rabbit Anti-CH25H, Cholesterol 25-Monooxygenase, EC 1.14.99.38, H25OH, C25H
    Purification
    This affinity purified antibody is directed against human CH25H.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Anti-CH25H antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of human CH25H conjugated to Keyhole Limpet Hemocyanin (KLH).

    Immunogen Type: Conjugated Peptide

    Top Product
    Discover our top product CH25H Primary Antibody
  • Application Notes
    Application Note: Anti-CH25H Antibody has been tested in ELISA, WB, and IHC.  Expect a band at ~31.7 kDa in western blot using appropriate tissues or lysates.  Positive control used: Human Kidney Tissues in Immunohistochemistry. Immunohistochemistry Dilution: 1:100 Western Blot Dilution: 1:1,000 ELISA Dilution: 1:10,000 - 1:50,000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.92 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    CH25H (Cholesterol 25-Hydroxylase (CH25H))
    Alternative Name
    CH25H (CH25H Products)
    Background
    Background: CH25H (Cholesterol 25-Hydroxylase catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. CH25H plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for several lymphoid cells. It may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. Anti-CH25H Antibody is useful for researchers interested in Alzheimer's Disease, Neuroscience, and metabolism.
    Gene ID
    9023
    NCBI Accession
    NP_003947
    UniProt
    O95992
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