LacZ antibody

Catalog No. ABIN7565954

The Chicken Polyclonal anti- antibody has been validated for ELISA, WB and DB. It is suitable to detect in samples from E. coli.

Quick Overview for LacZ antibody (ABIN7565954)

Target: LacZ

Reactivity: E. coli

Host: Chicken

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Dot Blot (DB)

Product Details

Purpose: Beta Galactosidase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Beta Galactosidase [E.

Characteristics: Synonyms: chicken anti-Beta Galactosidase Antibody, chicken anti-Beta Gal Antibody, Beta Galactosidase [E.coli], BGAL, beta-galactosidase, beta-gal

Purification: This Anti-Beta Galactosidase Antibody is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with full length native Beta Galactosidase isolated from E.coli

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Beta-Gal (CHICKEN) Antibody has been tested for use in ELISA and immunoblotting. Although not tested, this antibody may also be useful for neutralization assays, immunohistochemistry, flow Cytometry, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity. Western Blot Dilution: 1:2,000-1:10,000 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: LacZ

Background: Background: Anti Beta Galactosidase Antibody recognizes the enzyme beta galactosidase, or β-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of β-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti-beta Galactosidase Antibody is ideal for investigators involved in enzyme research.

NCBI Accession: NP_414878

UniProt: P00722