Protein A antibody

Catalog No. ABIN7565957

The Chicken Polyclonal anti-Protein A antibody has been validated for ELISA, WB and DB. It is suitable to detect Protein A in samples from Staphylococcus aureus.

Quick Overview for Protein A antibody (ABIN7565957)

Target: Protein A

Reactivity: Staphylococcus aureus

Host: Chicken

Clonality: Polyclonal

Conjugate: This Protein A antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Dot Blot (DB)

Product Details anti-Protein A Antibody

Purpose: Protein A Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus] and anti-Chicken Serum.

Characteristics: Synonyms: chicken anti-Protein A Antibody, chicken anti Protein A, chicken anti PA

Purification: This Anti-Protein A Antibody is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with Protein A  [Staphylococcus aureus].

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-PROTEIN A (CHICKEN) Antibody has been tested by western blotting and suitable for ELISA. Although not tested, this antibody may also be useful for neutralization assays, immunohistochemistry, flow Cytometry, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity. Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:500 - 1:2,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Protein A

Target: Protein A

Background: Background: Protein A is 56 kDA surface protein found in the cell wall of Staphylococcus aureus. Protein A has a high affinity to Human IgG, Mouse IgG and others and is therefore suitable for purification and detection of immunoglobulins.