IL1R2 antibody

Catalog No. ABIN7565967

The Rabbit Polyclonal anti-IL1R2 antibody (ABIN7565967) specifically detects IL1R2 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for IL1R2 antibody (ABIN7565967)

Target: IL1R2 (Interleukin 1 Receptor, Type II (IL1R2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1R2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IL1R2 Antibody

Purpose: IL-1 Receptor Type II Antibody

Cross-Reactivity (Details): The antibody is directed against the extracellular domain of the human IL-1R type II, but precipitates the intact receptor expressed on human cells.

Characteristics: Synonyms: rabbit anti-IL-1 Receptor Type II Antibody, rabbit anti-interleukin-1 receptor beta type II antibody, IL1R Beta antibody, Il1r-2 antibody, IL1R2 antibody, IL1RB antibody, Interleukin 1 receptor beta type II antibody

Purification: This is an IgG preparation of whole rabbit serum purified by Protein A chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1R type II.

Immunogen Type: Recombinant Protein

Application Details

Application Notes: Application Note: This IgG fraction antibody of anti-Human IL-1 Receptor Type II (IL-1RII) has been tested for use in ELISA and Western Blot and is suitable for use in radioimmunoassays, flow cytometry and immunoprecipitation. Reactivity in other immunoassays is unknown.  IL-1RII has been termed p68 or CD121 in some laboratories.  IL-1RII has been shown to bind IL-1, IL-1a and IL-1b. This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (code # 611-1302) and TMB as a substrate.  A working dilution range of 1:200 to 1:400 is suggested for immunoblot. This product has been assayed by ELISA against soluble (extracellular) IL-1RII using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature.  A working dilution range of 1:1,000 to 1:2000 is suggested for ELISA.  For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. A dilution range of 1:400 to 1:800 is suggested for immunoprecipitation.  For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background.  A dilution of 1:8,000 is suggested for radioimmunoassay.  Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for IL1R2

Target: IL1R2 (Interleukin 1 Receptor, Type II (IL1R2))

Alternative Name: IL1R2

Background: Background: Interleukin 1 receptor type II binds to IL-1 alpha, IL-1 beta and IL1R1/IL1RA. Anti-IL-1 Receptor Type II antibody is ideal for investigators involved in Immunology and Stem Cell research.

Gene ID: 7850

UniProt: P27930

Pathways: NF-kappaB Signaling