ABI1 antibody (C-Term)

Catalog No. ABIN7565970

This anti-ABI1 antibody is a Rabbit Polyclonal antibody detecting ABI1 in WB and ELISA. Suitable for Mouse.

Quick Overview for ABI1 antibody (C-Term) (ABIN7565970)

Target: ABI1 (Abl-Interactor 1 (ABI1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ABI1 Antibody

Binding Specificity: C-Term

Purpose: Abi1 Antibody

Cross-Reactivity (Details): Anti-Abi1 is directed against mouse Abi1.

Characteristics: Synonyms: rabbit anti-Abi1 Antibody, Abi-1, Abi 1, Abl Interactor 1, Eps8 SH3 Domain-Binding Protein, Abl-Binding Protein, Abelson Interactor 1, E3B1, Spectrin SH3 Domain Binding Protein 1, Nap1-binding protein, SSH3BP1

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to near c-terminal of the mouse Abi-1 protein.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes: Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 65 kDa in size corresponding to Abi1 by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 5.0 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.07 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for ABI1

Target: ABI1 (Abl-Interactor 1 (ABI1))

Alternative Name: Abi1

Background: Background: Anti-Abi1 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Abi1 gene has been suggested to have a role in inhibiting cell growth and encodes an adaptor protein in the Abelson-interactor family. The protein is involved in signal transduction, actin regulation and cytoskeletal remodeling. Studies suggest that Abi1 is a member of the WAVE complex and a dysfunctional complex may allow for the progression of prostate cancer. Anti-Abi1 is ideal for researchers interested in melanoma, colon and breast cancer and other cancer antibody research.

Gene ID: 11308

NCBI Accession: NP_001070658

UniProt: Q8CBW3

Pathways: RTK Signaling, Response to Water Deprivation, ER-Nucleus Signaling