CLIC1 antibody

Catalog No. ABIN7565990

The Rabbit Polyclonal anti-CLIC1 antibody has been validated for WB, ELISA and IP. It is suitable to detect CLIC1 in samples from Human.

Quick Overview for CLIC1 antibody (ABIN7565990)

Target: CLIC1 (Chloride Intracellular Channel 1 (CLIC1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLIC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-CLIC1 Antibody

Purpose: CLIC1 Antibody

Cross-Reactivity (Details): This antibody is specific for human CLIC1.

Characteristics: Synonyms: Rabbit Anti-CLIC1 Antibody, Chloride Intracellular Channel 1, Regulatory Nuclear Chloride Ion Channel Protein, Chloride Intracellular Channel Protein 1, Nuclear Chloride Ion Channel 27, Chloride Channel ABP, NCC27, HRNCC, G6, Nuclear Chloride Ion Channel Protein, RNCC Protein, P64CLCP

Purification: This product was Protein A purified from monospecific antiserum by chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with human CLIC1 recombinant protein.

Immunogen Type: Recombinant Protein

Application Details

Application Notes: Application Note: Anti-CLIC1 has been tested in ELISA, Western Blot, and Immunoprecipitation. Expect a band at ~27kda in western blot using appropriate tissues and lysates. Positive controls used in western blot include CLIC1 overexpressing HEK293, WM-115, and HL-60 whole cell lysates. Western Blot Dilution: 1 μg/mL Immunoprecipitation Dilution: User Optimized ELISA Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.06 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CLIC1

Target: CLIC1 (Chloride Intracellular Channel 1 (CLIC1))

Alternative Name: CLIC1

Background: Background: CLIC1 (Chloride Intracellular Channel 1) can insert into membranes and form chloride ion channels. Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH , and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family, the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity. This channel activity depends on the pH . Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle. Anti-CLIC1 is useful for researchers interested in Blackwater Fever and Penicilliosis diseases, along with Cancer, Neuroscience, and activation of cAMP-Dependent PKA.

Gene ID: 1192

NCBI Accession: NP_001274522

UniProt: O00299