Recombinant TNFSF13 antibody

Catalog No. ABIN7566473

The Mouse Monoclonal anti-TNFSF13 antibody has been validated for ELISA, IP and Func. It is suitable to detect TNFSF13 in samples from Mouse.

Quick Overview for Recombinant TNFSF13 antibody (ABIN7566473)

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFSF13 antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP), Functional Studies (Func)

Clone: Apry-1-1

Product Details anti-TNFSF13 Antibody

Purpose: anti-APRIL (mouse), mAb (rec.) (blocking) (Apry-1-1) (Fc LAEA-PG) (preservative free)

Characteristics:

Recombinant Antibody. Recognizes mouse APRIL. Does not recognize human APRIL and mouse/human BAFF. Source: HEK293. Isotype: Mouse IgG2blambda. Immunogen: Mouse APRIL (aa 98-232). Applications: ELISA, FUNC (Blocking), IP. Inhibits the binding of mouse APRIL to BCMA and TACI. Works in vitro and in vivo. APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth and is involved in monocyte/macrophage-mediated immunological processes. anti-APRIL (mouse), mAb (rec.) (Blocking) (Apry-1-1) (Fc LAEA-PG) (preservative free) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc mouse portion with mutations LAEA-PG and then produced in mammalian cells to generate an IgG like scFv-Fc (LAEA-PG) fusion protein. Mutations LAEA-PG inhibit binding to FcgammaRs and C1q while FcRn binding and Fc stability remain unaffected.

APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth and is involved in monocyte/macrophage-mediated immunological processes. anti-APRIL (mouse), mAb (rec.) (Blocking) (Apry-1-1) (Fc LAEA-PG) (preservative free) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc mouse portion with mutations LAEA-PG and then produced in mammalian cells to generate an IgG like scFv-Fc (LAEA-PG) fusion protein. Mutations LAEA-PG inhibit binding to FcgammaRs and C1q while FcRn binding and Fc stability remain unaffected.

Endotoxin Level: <0.001EU/μg purified protein (LAL-test, Lonza)

Immunogen: Mouse APRIL (aa 98-232).

Isotype: IgG2b lambda

Biological Activity Comment: Inhibits the binding of mouse APRIL to BCMA and TACI. Works in vitro and in vivo.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS.

Preservative: Without preservative

Handling Advice: After opening, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

Stable for at least 1 month after receipt when stored at +4°C.

Stable for at least 1 year after receipt when stored at -20°C.

Target Details for TNFSF13

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Alternative Name: APRIL

UniProt: Q9D777

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response