Double-Stranded RNA (dsRNA) antibody

Catalog No. ABIN7567105

This Mouse Monoclonal antibody specifically detects Double-Stranded RNA in ELISA and IF.

Quick Overview for Double-Stranded RNA (dsRNA) antibody (ABIN7567105)

Target: Double-Stranded RNA (dsRNA)

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF)

Clone: K2

Product Details

Purpose: Mouse anti double-stranded RNA (K2)

Specificity: The mAb K2 recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp dsRNA. Recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (4050 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognised by K2. As described by Schönborn et al. K2 binds with high avidity to all dsRNAs investigated.

Characteristics: K2 anti-dsRNA monoclonal antibody has an IgM isotype. K2 has primarily been used in ELISA and sandwich ELISA. Generally, K2 can be used in applications where an anti-dsRNA antibody with an isotype other than IgG (IgG2a) is required.The mAb K2 recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp dsRNA. Recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (4050 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognised by K2. As described by Schönborn et al. K2 binds with high avidity to all dsRNAs investigated.

Purification: Undiluted hybridoma supernatant

Immunogen: Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate the hybridoma clone.

Isotype: IgM kappa

Application Details

Application Notes: MAb K2 is primarily used for a sandwich ELISA to detect and quantitate (after calibration) dsRNA (see Schönborn et al.). For this application it should be diluted with PBS. It may also be advantageous to use K2 for immunofluorescence studies. The optimum working dilution of the antibody for any specific application should be established by titration. Not for use for clinical purposes. For in vitro use only.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Culture supernatant (RPMI, 5% fetal calf serum). No preservative is added.

Storage: -20 °C,-80 °C

Storage Comment: After delivery antibodies should be aliquoted and stored at -20 ° or -70 °C. After adding 10 mM sodium azide undiluted antibody can also be stored at +4 °C for a short period of time. For long term storage the antibody should be kept frozen. Repeated freezing/thawing cycles should be avoided.

Target Details

Target: Double-Stranded RNA (dsRNA)

Alternative Name: dsRNA

Target Type: Nucleotide

Background: Over the past decade our double-stranded RNA (dsRNA)antibodies have been used extensively to detect and characterise plant and animal viruses with dsRNA genomes or intermediates. In addition, the anti-dsRNA antibodies can be used as a diagnostic tool to detect pathogens, including detection in paraffin-embedded fixed tissue samples (Richardson et al. 2010).