ESR2 antibody (AA 201-300) (FITC)

Catalog No. ABIN725217

This anti-ESR2 antibody is a Rabbit Polyclonal antibody detecting ESR2 in WB, FACS, IF (cc) and IF (p). Suitable for Human, Mouse, Rat and Sheep.

Quick Overview for ESR2 antibody (AA 201-300) (FITC) (ABIN725217)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivity: Human, Mouse, Rat, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ESR2 antibody is conjugated to FITC

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-ESR2 Antibody (FITC)

Binding Specificity: AA 201-300

Cross-Reactivity: Human, Mouse, Rat, Sheep

Predicted Reactivity: Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ER-beta

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Alternative Name: Estrogen receptor beta

Background:

Synonyms: Erb, ESRB, ESTRB, NR3A2, ER-BETA, ESR-BETA, Estrogen receptor beta, Nuclear receptor subfamily 3 group A member 2, ESR2

Background: Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.

Gene ID: 2100

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling