DGKA antibody

Catalog No. ABIN7581173

Product Details anti-DGKA Antibody

Target: DGKA (Diacylglycerol Kinase, alpha 80kDa (DGKA))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This DGKA antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Sheep polyclonal antibody against diacylglycerol kinase α (DGKα)

Specificity: This antibody recognizes the alpha isoform of diacylglycerol kinase (DGK). DGK catalyzes a reaction terminating the protein kinase C mediated signaling by converting diacylglycerol (DAG) to phosphatidic acid (PA), a signaling lipid which is involved in DNA synthesis, phospholipid synthesis, and regulation of several enzymes.

Cross-Reactivity (Details): Shown to recognize an 80-85kDa recombinant DGKα protein with non-specific cross activity to wild-type DGK ζ protein.

Purification: Serum

Immunogen: peptide = MAKERGLISPSDFAQL

Isotype: IgG

Application Details

Application Notes: WB – 1:1000 Other in vitro and cellular applications are possible using this antibody, but have not been verified.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01% thiomersal

Storage: 4 °C,-20 °C

Storage Comment: Store at 4 °C for up to 30 days. Aliquot and store at -20 °C if longer storage is necessary. Antibody is stable for at least 2 years when stored at -20 °C. Avoid repeated freeze/thaw cycles.

Target Details for DGKA

Target: DGKA (Diacylglycerol Kinase, alpha 80kDa (DGKA))

Alternative Name: DGK Alpha (DGKA Products)

Background: DGK catalyzes a reaction terminating the protein kinase C (PKC)mediated signaling by converting diacylglycerol (DAG) to phosphatidic acid (PA), a signaling lipid which is involved in DNA synthesis, phospholipid synthesis, and regulation of several enzymes. DAG is an intermediate in phospholipid synthesis which functions as an allosteric activator of PKC. The alpha isoform is a type I DGKs, and has slightly different calcium-responsiveness.