Apelin Receptor antibody (Extracellular)

Catalog No. ABIN7581824

The Rabbit Polyclonal anti-Apelin Receptor antibody has been validated for WB, IHC, IF and FACS. It is suitable to detect Apelin Receptor in samples from Rat.

Quick Overview for Apelin Receptor antibody (Extracellular) (ABIN7581824)

Target: Apelin Receptor (APLNR)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Apelin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Apelin Receptor Antibody

Binding Specificity: AA 4-17, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Apelin Receptor

Sequence: DGYNYYGADN QSEC

Specificity: Extracellular, N-terminus

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Apelin/APJ Receptor (extracellular) Antibody (ABIN7581824) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize Apelin receptor from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: DGYNYYGADNQSEC, corresponding to amino acid residues 4-17 of rat apelin receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Apelin Receptor

Target: Apelin Receptor (APLNR)

Alternative Name: APLNR

Background: APLNR, Angiotensin receptor-like 1, AGTRL1, G-protein coupled receptor APJ, GPCR34,The apelin receptor (APJ) is a member of the G-protein coupled receptor gene family, encoded by the APLNR gene. The apelin receptor and its endogenous ligand, apelin, have been suggested to act as key mediators of physiological responses to multiple homeostatic actions, including cardiovascular control, water balance, hypothalamic-pituitary-adrenal (HPA) axis regulation and metabolic homeostasis.Apelin receptor structure contains seven hydrophobic transmembrane domains, with several sites for phosphorylation by protein kinase A, and sites for palmitoylation and glycosylation. The N-terminal glycosylation is implicated in the receptor's expression, stability, correct folding of the protein and ligand binding. The C-terminal palmitoylation has been reported to play a role in membrane association. These modifications can influence the internalization, dimerization and ligand binding of the receptor. Human apelin receptor shares 92 % homology with mouse receptor and 90 % homology with rat receptor.Apelin expression can be detected in several brain regions including the human caudate nucleus, corpus callosum, hippocampus, substantia nigra, subthalamic nucleus, medulla and spinal cord. Apelin receptor is also expressed in the spleen, placenta and cardiovascular tissues and small amounts in the small intestine, lung, stomach, colonic mucosa and ovary.

Gene ID: 83518

UniProt: Q9JHG3