BDKRB1 antibody (Extracellular)

Catalog No. ABIN7581830

The Rabbit Polyclonal anti-BDKRB1 antibody has been validated for WB, FACS and LCI. It is suitable to detect BDKRB1 in samples from Mouse.

Quick Overview for BDKRB1 antibody (Extracellular) (ABIN7581830)

Target: BDKRB1 (Bradykinin Receptor B1 (BDKRB1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BDKRB1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-BDKRB1 Antibody

Binding Specificity: AA 202-214, Extracellular

Purpose: A Rabbit Polyclonal Antibody to B1 Bradykinin Receptor

Sequence: (C)HEAWHFVRMV ELN

Specificity: Extracellular, 2nd loop.

Predicted Reactivity: Rat - 12 out of 13 amino acid residues identical Not recommended for human samples

Characteristics: Anti-B1 Bradykinin Receptor (BDKRB1) (extracellular) Antibody (ABIN7581830) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize B1 Bradykinin Receptor from mouse and rat samples. The antibody won't recognize human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)HEAWHFVRMVELN, corresponding to amino acid residues 202 - 214 of mouse BDKRB1 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400-1:500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for BDKRB1

Target: BDKRB1 (Bradykinin Receptor B1 (BDKRB1))

Alternative Name: BDKRB1

Background: BK-1 receptor, BKRB1, B1R, BKR1,Kinins are small peptides rapidly produced following tissue injury that serve as important modulators of inflammation and pain. In the periphery, the actions of kinins include vasodilatation, increased vascular permeability, stimulation of immune cells, and induction of pain. Kinins in the central nervous system (CNS) appear to initiate a similar cascade of events leading to neural tissue damage as well as long lasting disturbances affecting blood-brain barrier function.1Kinins such as Bradykinin (BK), Lys-BK, desArg9-BK, and Lys-desArg9-BK exert their action via two distinct receptors: the B1 Bradykinin receptor (BKRB1) and the B2 Bradykinin receptor (BKRB2). The desArg9-BK and Lys-desArg9-BK peptides activate BKRB1 while BK and Lys-BK operate by activating BKRB2.2 Both BKRB1 and BKRB2 are members of the seven-transmembrane domain, G protein-coupled receptor (GPCR) superfamily and share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Expression of BKRB1is inducible upon various types of tissue injury and by inflammatory mediators such as bacterial lipopolysaccharide (LPS) and cytokines. BKRB1 was long considered not to be expressed in healthy tissues. However, recent work has demonstrated a low level of expression of BKRB1 in the CNS of rodent and primates. In contrast, BKRB2 is constitutively expressed on various cell types.3 BKRB1 represents a potential therapeutic target for treatment of inflammatory disorders and cardiovascular diseases.

Gene ID: 12061

UniProt: Q61125

Pathways: ACE Inhibitor Pathway