CHRM3 antibody (Intracellular)

Catalog No. ABIN7581850

This Guinea Pig Polyclonal antibody specifically detects CHRM3 in WB and IHC. It exhibits reactivity toward Rat.

Quick Overview for CHRM3 antibody (Intracellular) (ABIN7581850)

Target: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This CHRM3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CHRM3 Antibody

Binding Specificity: AA 461-479, Intracellular

Purpose: A Guinea Pig Polyclonal Antibody to Acetylcholine receptor muscarinic 3

Sequence: (C)TLAKRFALKT RSQITKRKR

Specificity: 3rd intracellular loop

Predicted Reactivity: Human,mouse,bovine,pig - identical

Characteristics: Guinea Pig Anti-CHRM3 Antibody (ABIN7581850) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CHRM3 from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-CHRM3 (ABIN7043063, ABIN7044588 and ABIN7044589) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)TLAKRFALKTRSQITKRKR, corresponding to amino residues 461-479 of rat M3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7235122)

Blocking Peptide: (ABIN7235122)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CHRM3

Target: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Alternative Name: CHRM3

Background: Cholinergic receptor muscarinic 3, Acetylcholine receptor muscarinic 3, M3 AChR,The action of the neurotransmitter acetylcholine is mediated through two types of receptors, the ionotrophic nicotinic receptors and the metabotrophic muscarinic receptors. The muscarinic receptors belong to the superfamily of 7-TM G-protein-coupled receptors. Five subtypes of muscarinic receptors have been cloned and named m1-m5.1-2The muscarinic receptors are widely distributed throughout the body, but are predominantly expressed within the parasympathetic nervous system and exerts both excitatory and inhibitory control over central and peripheral tissues.1-2 The m3 receptor is expressed in brain, heart, urinary bladder, salivary gland and airway smooth muscle.3Muscarinic receptors participate in a number of physiologic functions such as regulation of heart rate, muscle contraction, cognition, sensory processing and motor control.1 They also participate in learning and memory processing.4-5 The m1 receptors are the most abundant muscarinic subtype in the cortex and striatum. m1 receptors were also localized in the myenteric plexus where they function as autoreceptors to enhance the release of Ach from the nerves.6-7 m2, found in heart, is coupled to and mediates the slowing of heart rate in response to acetylcholine.3,8 m3 controls smooth muscle contraction and its stimulation causes secretion from glandular tissues.9Involvement of muscarinic receptors in apoptosis and cell growth has been reported.10-14 m1, m3 and m5, which are coupled to Gq/11 proteins, can protect cells from undergoing apoptosis induced by DNA damage. The signaling mechanism that mediates this anti-apoptotic response is still poorly understood. However, it was recently reported that a poly-basic motif in the C-terminus tail of the m3, m1 and m5 receptors is an essential element for the anti-apoptotic response of those receptors.10Muscarinic receptors are also capable of promoting cell proliferation in many cell types, however, the signaling pathways are yet, unclear.11-14 In the literature, m3 was reported to be involved in mitogenesis and proliferation of different cell types. Recently, it was reported that activation of the m3 muscarinic receptor induces significant proliferation of colon and prostate cancer cells.11-14

Gene ID: 24260

UniProt: P08483

Pathways: Synaptic Membrane