CNR1 antibody (Extracellular, N-Term)

Catalog No. ABIN7886238

The Guinea Pig Polyclonal anti-CNR1 antibody is suitable to detect CNR1 in samples from Rat. It has been validated for WB and IHC.

Quick Overview for CNR1 antibody (Extracellular, N-Term) (ABIN7886238)

Target: CNR1 (Cannabinoid Receptor 1 (CNR1))

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This CNR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CNR1 Antibody

Binding Specificity: AA 84-99, Extracellular, N-Term

Purpose: A Guinea Pig Polyclonal Antibody to Cannabinoid Receptor 1 (extracellular)

Sequence: NKSLSSFKEN EENIQC

Predicted Reactivity: Human - identical, mouse - 15,16 amino acid residues identical

Characteristics: Guinea Pig Anti-Cannabinoid Receptor 1 (extracellular) Antibody (ABIN7581863) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CB1R from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Cannabinoid Receptor 1 (extracellular) Antibody (ABIN7043023, ABIN7044155 and ABIN7044156) raised in rabbits. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Purification: Affinity purified on immobilized antigen.

Immunogen: NKSLSSFKENEENIQC, corresponding to amino acid residues 84-99 of rat CB1 receptor

Isotype: IgG

Application Details

Application Notes:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235028)

Blocking Peptide: (ABIN7235028)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CNR1

Target: CNR1 (Cannabinoid Receptor 1 (CNR1))

Alternative Name: CNR1

Background:

Synonyms: CB1, CB-R, CB1R, CANN6, Brain-type cannabinoid receptor, Central cannabinoid receptor, CNR1

Description: Cannabinoids have been used in Eastern medicine for many years as pain relievers.1 Δ9-tetrahydrocannabinol (THC), the major psychoactive compound in marijuana and hashish, has been shown to interact with two specific cannabinoid receptors: cannabinoid receptor 1 (CB1 receptor) and cannabinoid receptor 2 (CB2 receptor).2 The cannabinoid receptors can be distinguished by their amino acid sequences, signaling mechanisms, and tissue distributions.2 Both receptors belong to the G-protein coupled receptor (GPCR) superfamily. CB1 was shown to modulate several Ca2+ and K+ ion channels.2,3CB1 is primarily expressed in the central nervous system. However, expression of CB1 is also detected in the peripheral terminals, in non-neuronal peripheral tissues such as uterus, testes, spleen, as well as in cells of the immune system.3,4CB1 is implicated in many cellular functions such as neurotransmitter release, pain relief, cancer, and obesity.5,6 Growth inhibition of tumor cells was demonstrated following mixed CB1/CB2 agonist treatment in both prostate and non-melanoma skin cancers.5,6 Through their interaction with CB1, cannabinoid compounds stimulate appetite for sweets and palatable foods in particular, making CB1 an attractive therapeutic target for the treatment of obesity and eating disorders.7

Gene ID: 25248

UniProt: P20272

Pathways: Feeding Behaviour