DSCAM antibody (Extracellular, N-Term)

Catalog No. ABIN7581866

The Rabbit Polyclonal anti-DSCAM antibody is suitable to detect DSCAM in samples from Mouse. It has been validated for WB, IHC and LCI.

Quick Overview for DSCAM antibody (Extracellular, N-Term) (ABIN7581866)

Target: DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DSCAM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Product Details anti-DSCAM Antibody

Binding Specificity: AA 361-377, Extracellular, N-Term

Purpose: A Rabbit Polyclonal antibody to L1CAM (extracellular)

Sequence: (C)RITGLNHANL IMDHMVK

Predicted Reactivity: Rat - identical,Human - 15 out of 17 amino acid residues identical

Characteristics: Anti-DSCAM (extracellular) Antibody (ABIN7581866) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize DSCAM from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RITGLNHANLIMDHMVK, corresponding to amino acid residues 361 - 377 of mouse DSCAM

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for DSCAM

Target: DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))

Alternative Name: DSCAM

Background:

Synonyms: Down Syndrome Cell Adhesion Molecule, DS Cell Adhesion Molecule, CHD2

Description: Down syndrome cell adhesion molecule, DSCAM, also known as CHD2, is a member of the immunoglobulin superfamily of cell adhesion molecules (Ig-CAMs). DSCAM is classified as a type I membrane protein with an extracellular domain consisting of ten immunoglobulin (Ig) and six fibronectin type III (FNIII) repeats and an intracellular domain that does not contain any identifiable motifs.1The gene coding for the DSCAM protein is located on the human chromosome 21, specifically in the so-called Down syndrome critical region (DSCR), the trisomy of which is considered a determinant for the intellectual disability associated with Down's syndrome. Therefore, the potential involvement of DSCAM in the developing neural system, has received considerable attention.2,3DSCAM is widely expressed in the vertebrate central nervous system where it plays a crucial role in neural development. DSCAM was found to have a critical role for the development of neural circuits, playing a role in processes such as axon guidance, dendritic arborization, and synapse formation. 2-5DSCAM is involved in axon guidance, a process by which developing neurons extend their axons to establish specific connections with target cells. DSCAM serves as a receptor for Netrin-1, a member of a conserved family of secreted proteins that promote axon outgrowth and guide growth cone navigation.2,6The molecular mechanisms by which DSCAM contributes to autism spectrum disorders (ASDs), is not clear, but it may involve altered synaptic transmission, probably through its interaction with other adhesion molecules such as neuroligins (NLGNs) and neurexin1 (NRXN1). In fact, deletion of DSCAM in neurons results in abnormal spine maturation, altered synaptic transmission, as well as autism-like behaviors.5

Gene ID: 13508

UniProt: Q9ERC8

Pathways: Regulation of Cell Size