PAR1 antibody (Extracellular)

Catalog No. ABIN7581870

The Rabbit Polyclonal anti-PAR1 antibody (ABIN7581870) specifically detects PAR1 in WB, FACS and LCI. The antibody is reactive with Mouse samples.

Quick Overview for PAR1 antibody (Extracellular) (ABIN7581870)

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAR1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-PAR1 Antibody

Binding Specificity: AA 66-80, Extracellular

Purpose: A Rabbit Polyclonal Antibody to PAR1 (F2R) (extracellular)

Sequence: (C)KNESVLLEGR AVYLN

Specificity: Extracellular, N-terminus.

Predicted Reactivity: Rat - 13 out of 15 amino acid residues identical It won't recognize human samples

Characteristics: Anti-PAR1 (F2R) (extracellular) Antibody (ABIN7581870) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize PAR1 from mouse and rat samples. The antibody won't recognize human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KNESVLLEGRAVYLN, corresponding to amino acid residues 66 - 80 of mouse F2R

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:400

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for PAR1

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Alternative Name: F2R

Background: Proteinase-activated receptor 1, Coagulation factor II receptor, CF2R,Protease-activated receptor 1 (PAR-1) belongs to a family of four G protein-coupled receptors (PAR1-4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their name. In this novel modality of activation, a specific protease cleaves the PAR receptor within a defined sequence in its extracellular N-terminal domain. This results in the creation of a new N-terminal tethered ligand, which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several signal transduction pathways.1-3Different PARs are activated by different proteases. Hence, PAR-1 is activated by thrombin (and is in fact also known as the thrombin receptor), as are PAR-3 and PAR-4, while PAR-2 is activated by trypsin.1-3 PAR-1 can be also cleaved and activated by other proteases such as plasmin, Factor Xa, cathepsin G, and others.The intramolecular nature of PAR activation and the continuous presence of the tethered ligand that cannot diffuse away imply the existence of several mechanisms for the rapid termination of PAR signaling. Indeed, following receptor activation, there is rapid phosphorylation of the C-terminal end of the receptor, followed by receptor internalization and degradation. In addition, several proteases can cleave away the tethered ligand, thereby "disarming" the PAR.1-3PAR-1 signals through several G proteins including Gaq, Gai, and Ga12/13, resulting in the activation of several transduction pathways including intracellular Ca2+ mobilization, Rho and Rac signaling, and MAPK activation.1-3PAR-1 is expressed in several cell types including platelets, leukocytes, vascular endothelial cells, gastrointestinal epithelial cells, myocytes, and neurons. The best studied physiological function of PAR-1 is its involvement in the coagulation cascade. Thrombin, the preeminent ligand of PAR-1, activates the receptor on the surface of platelets, hence inducing platelet aggregation, granular secretion, and procoagulant activity. PAR-1 also plays a crucial role in vascular ontogenesis. Accordingly, PAR-1 knockout mice show bleeding at multiple sites and usually die at mid-gestation.1-3PAR-1 also plays important roles in tumor growth and metastasis. PAR-1 is upregulated in several human cancers as are several proteases such as plasmin and matrix metalloproteases (MMPs) that act as PAR-1 ligands, thereby creating an autocrine loop. PAR-1 activation in cancer cells transmits mitogenic signals through the activation of the erk1/2 pathway and is involved in tumor spread via its pro-angiogenic activity.4

Gene ID: 14062

UniProt: P30558

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus