GPR108 antibody (Extracellular)

Catalog No. ABIN7581883

This Rabbit Polyclonal antibody specifically detects GPR108 in WB, IHC, FACS and LCI. It exhibits reactivity toward Mouse.

Quick Overview for GPR108 antibody (Extracellular) (ABIN7581883)

Target: GPR108 (G Protein-Coupled Receptor 108 (GPR108))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR108 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-GPR108 Antibody

Binding Specificity: AA 124-137, Extracellular

Purpose: A Rabbit Polyclonal antibody to GPR108 (extracellular)

Sequence: (C)KDLQVQVRKY GEQK

Specificity: Extracellular, N-term.

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-GPR108 (extracellular) Antibody (ABIN7581883) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize GPR108 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KDLQVQVRKYGEQK, corresponding to amino acid residues 124 - 137 of mouse GPR108

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR108

Target: GPR108 (G Protein-Coupled Receptor 108 (GPR108))

Alternative Name: GPR108 

Background: G-Protein Coupled Receptor 108, Lung Seven Transmembrane Receptor 2, LUSTR2,G protein coupled receptors (GPCR or GPR) comprise a large superfamily of receptors characterized by a seven transmembrane domain structure and an ability to activate intracellular transducer G proteins. Over 800 GPCRs in five main families have been identified in eukaryotes on the basis of genomic sequence analysis1,2. These receptors can be activated by hormones, neurotransmitters, odorants, light, or pheromones. Whereas the endogenous agonist is remains unclear or in dispute2.GPCRs mediated the signaling of extracellular stimuli to intracellular responses via activation of heterotrimeric G proteins and their subsequent interaction with effector proteins. GPCRs have been implicated in a number of physiological functions including development of anxiety, behavior, homeostasis, cognition, appetite, and drug addiction3.The GPCR family contains a diverse group of cell-surface mediators of signal transduction, its size exceeding other cell-surface protein receptors including the tyrosine kinase receptors, guanylyl cyclase receptors and ligand-gated ion channels4.GPR108 (LUSTR2), has a large extracellular domain of an amino-terminal hydrophobic signal peptide sequence, and a carboxy-terminal seven-transmembrane domain5. Previous studies showed that GPR108 is linked to nuclear factor κB (NF-κB) signaling6 and regulates and inflammatory immune response and cancer signaling7.Recently, GPR108 has been identified as a highly conserved cellular entry factor for Adeno-associated virus (AAV), a widely used and clinically approved gene therapy vector8.

Gene ID: 78308

UniProt: Q91WD0